catalog number :
MBS701357
products type :
ELISA Kit
products full name :
Mouse monocyte chemotactic protein 2, MCP-2 ELISA Kit
products short name :
chemokine (C-C motif) ligand 8
products name syn :
Mouse monocyte chemotactic protein 2; MCP-2 ELISA kit; HC14; MCP-2; MCP2; SCYA10; SCYA8; monocyte chemoattractant protein 2; monocyte chemotactic protein 2; small inducible cytokine A8; small inducible cytokine subfamily A (Cys-Cys); member 8 (monocyte che; chemokine (C-C motif) ligand 8
other names :
C-C motif chemokine 8; C-C motif chemokine 8; C-C motif chemokine 8; small inducible cytokine A8; small-inducible cytokine A8; monocyte chemotactic protein 2; monocyte chemoattractant protein 2; monocyte chemoattractant protein-2; chemokine (C-C motif) ligand 8; Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; MCP-2; Small-inducible cytokine A8
products gene name :
CCL8
other gene names :
Ccl8; Ccl8; HC14; Mcp2; MCP-2; Scya8; AB023418; 1810063B20Rik; Mcp2; Scya8; MCP-2
uniprot entry name :
CCL8_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse MCP-2. No significant cross-reactivity or interference between mouse MCP-2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 pg/ml -400 pg/ml. Sensitivity: The minimum detectable dose of mouse MCP-2 is typically less than 1.56 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MCP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_067418.1
ncbi gb acc num :
NM_021443.3
ncbi mol weight :
11,017 Da
ncbi pathways :
Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (672435); Chemokine Signaling Pathway (96864); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460)
uniprot summary :
Function: Chemotactic factor that attracts monocytes. This protein can bind heparin
size4 :
10x96-Strip-Wells