catalog number :
MBS701356
products type :
ELISA Kit
products full name :
Rabbit Complement 3, C3 ELISA Kit
products short name :
complement component 3
products name syn :
Rabbit Complement 3; C3 ELISA Kit; AHUS5; ARMD9; ASP; CPAMD1; acylation-stimulating protein cleavage product; complement component C3; complement component 3
other names :
complement C3 preproprotein; Complement C3; complement C3; complement component 3<; C3adesArg
other gene names :
C3; C3; ASP
uniprot entry name :
CO3_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of Rabbit C3. No significant cross-reactivity or interference between Rabbit C3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Competitive. Detection Range: 27.3 ng/ml-7000 ng/ml. Sensitivity: 27.3 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Complement. Protein Biological Process 3: Complement alternate pathway
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antibody preparation specific for C3. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated C3. The competitive inhibition reaction is launched between with HRP conjugated C3 and C3 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of C3 in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001035559.2
ncbi gb acc num :
NM_001040469.2
ncbi mol weight :
187,253 Da
ncbi pathways :
Activation Of C3 And C5 Pathway (1009150); Adaptive Immune System Pathway (1009080); Alternative Complement Activation Pathway (1009149); Chagas Disease (American Trypanosomiasis) Pathway (147808); Chagas Disease (American Trypanosomiasis) Pathway (147795); Class A/1 (Rhodopsin-like Receptors) Pathway (1008554); Complement Activation, Classical Pathway (920897); Complement And Coagulation Cascades Pathway (920919); Complement And Coagulation Cascades Pathway (84312); Complement And Coagulation Cascades Pathway (484)
uniprot summary :
Function: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates
size4 :
10x96-Strip-Wells