catalog number :
MBS701355
products type :
ELISA Kit
products full name :
Human SAA/LDL Complex ELISA Kit
products short name :
SAA/LDL Complex
products name syn :
Human SAA/LDL Complex ELISA kit; SAA/LDL Complex
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.156 ug/ml -10 ug/ml. Detection Wavelength: 450 nm. Sensitivity: 0.039 ug/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SAA/LDL complex. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for SAA/LDL complex and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain SAA/LDL complex, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SAA/LDL complex in the samples is then determined by comparing the O.D. of the samples to the standard curve.