ELISA/assay

Human Cluster of differentiation 3, CD3 ELISA Kit

MBS701346

96 Strip Wells

850 USD

ELISA Kit

Human Cluster of differentiation 3, CD3 ELISA Kit

Cluster of differentiation 3

Human Cluster of differentiation 3; CD3 ELISA Kit; Cluster of differentiation 3

cd3; CD3gamma/delta; CD3gamma/delta; Cd3 protein; T-cell surface glycoprotein CD3 gamma/delta chain homolog

CD3

cd3g; cd3; cd3g-a

P79951_XENLA

Human

167

This assay has high sensitivity and excellent specificity for detection of human CD3. No significant cross-reactivity or interference between human CD3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human cluster of differentiation 3 (CD3) concentrations in serum, plasma, tissue homogenates. Detection Range: 0.312 ng/ml-20 ng/ml.

Sensitivity: The minimum detectable dose of human CD3 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

1914807

CAA72995.1

P79951

18,897 Da

Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478!!MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!Salmonella Infection Pathway 375172!!Salmonella Infection Pathway 375149

96 Strip Wells

850 USD