ELISA/assay

human hemochromatosis type 2/uvenile-uman homolog, HFE2 LISA Kit ELISA Kit

MBS701344

48-Strip-Wells

510 USD

ELISA Kit

human hemochromatosis type 2/uvenile-uman homolog, HFE2 LISA Kit ELISA Kit

hemochromatosis type 2 (juvenile)

human hemochromatosis type 2/uvenile-uman homolog; HFE2 LISA Kit; HFE2A; HJV; JH; MGC23953; RGMC; RGM domain family; member C; haemojuvelin; hemochromatosis type 2 protein; hemojuvelin; hemochromatosis type 2 (juvenile)

hemojuvelin isoform b; Hemojuvelin; hemojuvelin; haemojuvelin; RGM domain family member C; repulsive guidance molecule c; hemochromatosis type 2 protein; hemochromatosis type 2 (juvenile); Hemochromatosis type 2 protein; RGM domain family member C

HFE2

HFE2; HFE2; JH; HJV; RGMC; HFE2A; HJV; RGMC

RGMC_HUMAN

Human

313

This assay has high sensitivity and excellent specificity for detection of Human HFE2. No significant cross-reactivity or interference between Human HFE2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Detection Range: 125 ng/ml-4000 ng/ml. Sensitivity: 62.5 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HFE2. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated antibody preparation specific for HFE2 and incubated. Then substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HFE2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

44662823

NP_660320.3

NM_145277.4

Q6ZVN8

45,080 Da

Axon Guidance Pathway (105688); BMP Receptor Signaling Pathway (137948); Developmental Biology Pathway (477129); Netrin-1 Signaling Pathway (160999)

The product of this gene is involved in iron metabolism. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. It could also represent the cellular receptor for hepcidin. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Defects in this gene are the cause of hemochromatosis type 2A, also called juvenile hemochromatosis (JH). JH is an early-onset autosomal recessive disorder due to severe iron overload resulting in hypogonadotrophic hypogonadism, hepatic fibrosis or cirrhosis and cardiomyopathy, occurring typically before age of 30. [provided by RefSeq, Jul 2008]

Function: Member of the repulsive guidance molecule (RGM) family. Involved in iron metabolism. Acts as a bone morphogenetic protein (BMP) coreceptor Enhancement of BMP signaling regulates hepcidin (HAMP) expression and iron metabolism May cooperate with hepcidin to restrict iron absorption in the gut. Could represent the cellular receptor for hepcidin.

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800