Mouse Tumor necrosis factor alpha, TNF-alpha ELISA Kit | MyBioSource MBS701337 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Tumor necrosis factor alpha, TNF-alpha ELISA Kit

catalog :

MBS701337

quantity :

48-Strip-Wells

price :

465 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701337

products type :

ELISA Kit

products full name :

Mouse Tumor necrosis factor alpha, TNF-alpha ELISA Kit

products short name :

tumor necrosis factor (TNF superfamily, member 2)

products name syn :

Mouse Tumor necrosis factor alpha; TNF-alpha ELISA KIT; DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein; TNF superfamily; member 2; TNF; macrophage-derived; TNF; monocyte-derived; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily; member 2)

other names :

tumor necrosis factor isoform 2; Tumor necrosis factor; tumor necrosis factor; cachectin; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

products gene name :

TNF

other gene names :

Tnf; Tnf; DIF; Tnfa; TNF-a; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; Tnfa; Tnfsf2; TNF-a; NTF; ICD1; ICD2

uniprot entry name :

TNFA_MOUSE

reactivity :

Mouse

sequence length :

219

specificity :

This assay has high sensitivity and excellent specificity for detection of Mouse TNF. No significant cross-reactivity or interference between Mouse TNF and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

For Sample For the quantitative determination of mouse tumor necrosis factor ? (TNF-?) concentrations in serum, plasma, cell culture supernates, tissue homogenates. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TNF-? has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-? present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNF-? is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-? bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

518831589

ncbi acc num :

NP_001265530.1

ncbi gb acc num :

NM_001278601.1

uniprot acc num :

P06804

ncbi mol weight :

25,896 Da

ncbi pathways :

Adipocytokine Signaling Pathway (83290); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198299); African Trypanosomiasis Pathway (194387); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83316); Allograft Rejection Pathway (535); Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167330)

ncbi summary :

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

uniprot summary :

Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

size1 :

48-Strip-Wells

price1 :

465 USD

size2 :

96-Strip-Wells

price2 :

655

size3 :

5x96-Strip-Wells

price3 :

2455

size4 :

10x96-Strip-Wells

price4 :

4595

more info or order :

MyBioSource product webpage