catalog number :
MBS701336
products type :
ELISA Kit
products full name :
Rat Bone morphogenetic protein 2, BMP-2 ELISA Kit
products short name :
bone morphogenetic protein 2
products name syn :
Rat Bone morphogenetic protein 2; BMP-2 ELISA Kit; BMP2A; ; bone morphogenetic protein 2
other names :
bone morphogenetic protein 2; Bone morphogenetic protein 2; bone morphogenetic protein 2
products gene name :
BMP2
other gene names :
BMP2; BMP2; BMP-2; BMP-2; BMP-2
uniprot entry name :
BMP2_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat BMP-2. No significant cross-reactivity or interference between rat BMP-2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml -500 pg/ml. Sensitivity: The minimum detectable dose of rat BMP-2 is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BMP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001076119.1
ncbi gb acc num :
NM_001082650.1
ncbi mol weight :
44,664 Da
ncbi pathways :
Adipogenesis Pathway (198832); BMP Signalling Pathway (545370); Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Heart Development Pathway (198802); Hedgehog Signaling Pathway (83063); Hedgehog Signaling Pathway (474); Id Signaling Pathway (198871)
uniprot summary :
Function: Induces cartilage and bone formation
size4 :
10x96-Strip-Wells