ELISA/assay

Rat Tyrosine Hydroxylase, TH ELISA Kit

MBS701326

48-Strip-Wells

565 USD

ELISA Kit

Rat Tyrosine Hydroxylase, TH ELISA Kit

tyrosine hydroxylase

Rat Tyrosine Hydroxylase (TH) ELISA Kit; DYT14; DYT5b; TYH; dystonia 14; tyrosine 3-monooxygenase; tyrosine hydroxylase

tyrosine 3-monooxygenase; Tyrosine 3-monooxygenase; tyrosine 3-monooxygenase; tyrosine 3-hydroxylase; tyrosine hydroxylase; Tyrosine 3-hydroxylase; TH

TH

Th; Th; The; TH

TY3H_RAT

Rat

498

This assay has high sensitivity and excellent specificity for detection of rat TH. No significant cross-reactivity or interference between rat TH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, tissue homogenates. Assay Type: Sandwich. Detection Range: 78 pg/ml -5000 pg/ml. Sensitivity: 19.5 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TH bound in the initial step. The color development is stopped and the intensity of the color is measured.

6981652

NP_036872.1

NM_012740.3

P04177

55,966 Da

Alcoholism Pathway (585585); Alcoholism Pathway (587116); Amine-derived Hormones Pathway (1010837); Amphetamine Addiction Pathway (547642); Amphetamine Addiction Pathway (550546); Biogenic Amine Synthesis Pathway (198489); Catecholamine Biosynthesis Pathway (1010838); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (434674); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (468235); Catecholamine Synthesis Pathway (198533)

catalyzes the rate limiting step in catecholamine biosynthesis; may be involved in response to hypoxia [RGD, Feb 2006]

Function: Plays an important role in the physiology of adrenergic neurons. Catalytic activity: L-tyrosine + tetrahydrobiopterin + O2 = L-dopa + 4a-hydroxytetrahydrobiopterin. Cofactor: Fe2+ ion. Enzyme regulation: Phosphorylation leads to an increase in the catalytic activity.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150