Human plasma kallikrein, KLKB1 ELISA Kit | MyBioSource MBS701309 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human plasma kallikrein, KLKB1 ELISA Kit

catalog :

MBS701309

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701309

products type :

ELISA Kit

products full name :

Human plasma kallikrein, KLKB1 ELISA Kit

products short name :

kallikrein B, plasma (Fletcher factor) 1

products name syn :

Human plasma kallikrein (KLKB1) ELISA kit; KLK3; PPK; Fletcher factor; kininogenin; plasma kallikrein B1; plasma kallikrein heavy chain; plasma kallikrein light chain; kallikrein B; plasma (Fletcher factor) 1

other names :

plasma kallikrein preproprotein; Plasma kallikrein; plasma kallikrein; kininogenin; Fletcher factor; plasma prekallikrein; plasma kallikrein heavy chain; plasma kallikrein light chain; kallikrein B, plasma (Fletcher factor) 1; Fletcher factor; Kininogenin; Plasma prekallikreinCleaved into the following 2 chains:Plasma kallikrein heavy chain; Plasma kallikrein light chain

products gene name :

KLKB1

other gene names :

KLKB1; KLKB1; PPK; KLK3; KLK3

uniprot entry name :

KLKB1_HUMAN

reactivity :

Human

sequence length :

638

specificity :

This assay has high sensitivity and excellent specificity for detection of human KLKB1. No significant cross-reactivity or interference between human KLKB1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 9.38 mU/ml -600 00 mU/ml. Sensitivity: 2.34 mU/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KLKB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLKB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLKB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLKB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

78191798

ncbi acc num :

NP_000883.2

ncbi gb acc num :

NM_000892.3

uniprot acc num :

P03952

ncbi mol weight :

71,370 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Blood Clotting Cascade Pathway (198840); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Hemostasis Pathway (106028); Intrinsic Pathway (106059)

ncbi summary :

Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma kallikrein therefore is composed of a heavy chain and a light chain held together by a disulphide bond. The heavy chain originates from the amino-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91 amino acids. Each repeat harbors a novel structure called the apple domain. The heavy chain is required for the surface-dependent pro-coagulant activity of plasma kallikrein. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. Catalytic activity: Cleaves selectively Arg- -Xaa and Lys- -Xaa bonds, including Lys- -Arg and Arg- -Ser bonds in (human) kininogen to release bradykinin. Enzyme regulation: Inhibited by SERPINA5.1 PublicationManual assertion based on experiment in:Ref.9

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage