Human Soluble Mesothelin Related Peptide, SMRP ELISA Kit | MyBioSource MBS701296 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Soluble Mesothelin Related Peptide, SMRP ELISA Kit

catalog :

MBS701296

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701296

products type :

ELISA Kit

products full name :

Human Soluble Mesothelin Related Peptide, SMRP ELISA Kit

products short name :

ATP-binding cassette, sub-family C (CFTR/MRP) , member 5

products name syn :

Human Soluble Mesothelin Related Peptide (SMRP) ELISA Kit; ABC33; DKFZp686C1782; EST277145; MOAT-C; MOATC; MRP5; SMRP; pABC11; ATP-binding cassette; sub-family C; member 5; canalicular multispecific organic anion transporter C; ATP-binding cassette; sub-family C (CFTR/MRP); member 5

other names :

multidrug resistance-associated protein 5 isoform 2; Multidrug resistance-associated protein 5; multidrug resistance-associated protein 5; ATP-binding cassette sub-family C member 5; multi-specific organic anion transporter C; canalicular multispecific organic anion transporter C; ATP-binding cassette, sub-family C (CFTR/MRP), member 5; ATP-binding cassette sub-family C member 5; Multi-specific organic anion transporter C; MOAT-C; SMRP; pABC11

products gene name :

ABCC5

other gene names :

ABCC5; ABCC5; MRP5; SMRP; ABC33; MOATC; MOAT-C; pABC11; EST277145; MRP5; MOAT-C

uniprot entry name :

MRP5_HUMAN

reactivity :

Human

sequence length :

208

specificity :

This assay has high sensitivity and excellent specificity for detection of human SMRP. No significant cross-reactivity or interference between human SMRP and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.312 pmol/ml - 20 pmol/ml. Sensitivity: The minimum detectable dose of human SMRP is typically less than 0.078 pmol/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SMRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SMRP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMRP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMRP bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

66529093

ncbi acc num :

NP_001018881.1

ncbi gb acc num :

NM_001023587.1

uniprot acc num :

O15440

ncbi mol weight :

160,660 Da

ncbi pathways :

ABC Transporters Pathway (83035); ABC Transporters Pathway (436); ABC-family Proteins Mediated Transport Pathway (106573); Disease Pathway (530764); Fluoropyrimidine Activity Pathway (198778); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Metabolism Pathway (645297); Hyaluronan Biosynthesis And Export Pathway (645299); Hyaluronan Metabolism Pathway (645298); MPS I - Hurler Syndrome Pathway (685537)

ncbi summary :

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that this protein provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl)adenine. This protein may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Alternative splicing of this gene has been detected; however, the complete sequence and translation initiation site is unclear. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Acts as a multispecific organic anion pump which can transport nucleotide analogs.1 PublicationManual assertion based on experiment in:Ref.4

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage