Human Xanthine Oxidase, XOD ELISA Kit | MyBioSource MBS701269 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Xanthine Oxidase, XOD ELISA Kit

catalog :

MBS701269

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701269

products type :

ELISA Kit

products full name :

Human Xanthine Oxidase, XOD ELISA Kit

products short name :

xanthine dehydrogenase

products name syn :

Human Xanthine Oxidase (XOD) ELISA Kit; XO; XOR; xanthene dehydrogenase; xanthine oxidase; xanthine oxidoreductase; xanthine dehydrogenase

other names :

xanthine dehydrogenase/oxidase; Xanthine dehydrogenase/oxidase; xanthine dehydrogenase/oxidase; xanthine oxidoreductase; xanthine dehydrogenase; Xanthine oxidoreductase; XOR

products gene name :

XDH

other gene names :

XDH; XDH; XO; XOR; XDHA; XD; XO; XOR

uniprot entry name :

XDH_HUMAN

reactivity :

Human

sequence length :

1333

specificity :

This assay has high sensitivity and excellent specificity for detection of Human XDH. No significant cross-reactivity or interference between Human XDH and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, urine, tissue homogenates. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to XOD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for XOD and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain XOD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of XOD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

91823271

ncbi acc num :

NP_000370.2

ncbi gb acc num :

NM_000379.3

uniprot acc num :

P47989

ncbi mol weight :

146,424 Da

ncbi pathways :

Caffeine Metabolism Pathway (82945); Caffeine Metabolism Pathway (308); Drug Metabolism - Other Enzymes Pathway (83033); Drug Metabolism - Other Enzymes Pathway (428); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Oxidative Stress Pathway (198916); Peroxisome Pathway (131226); Peroxisome Pathway (131126)

ncbi summary :

Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq, Jan 2014]

uniprot summary :

Function: Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).1 PublicationManual assertion based on experiment in:Ref.9. Catalytic activity: Hypoxanthine + NAD+ + H2O = xanthine + NADH.2 PublicationsManual assertion based on experiment in:Ref.4. Cofactor: Binds 2 2Fe-2S clusters.1 PublicationManual assertion based on experiment in:Ref.9. Enzyme regulation: Can be converted from the dehydrogenase form (D) to the oxidase form (O) irreversibly by proteolysis or reversibly through the oxidation of sulfhydryl groups 1 PublicationManual assertion based on experiment in:Ref.10

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage