ELISA/assay

Mouse cyclophilin A, CyPA ELISA Kit

MBS701267

48-Strip-Wells

510 USD

ELISA Kit

Mouse cyclophilin A, CyPA ELISA Kit

peptidylprolyl isomerase A (cyclophilin A)

Mouse cyclophilin A; CyPA ELISA Kit; CYPA; CYPH; MGC117158; MGC12404; MGC23397; PPIase A; T cell cyclophilin; cyclosporin A-binding protein; peptidyl-prolyl cis-trans isomerase A; peptidylprolyl isomerase A; rotamase A; peptidylprolyl isomerase A (cyclophilin A)

peptidyl-prolyl cis-trans isomerase A; Peptidyl-prolyl cis-trans isomerase A; peptidyl-prolyl cis-trans isomerase A; SP18; PPIase A; rotamase A; cyclophilin A; cyclosporin A-binding protein; peptidylprolyl isomerase A; Cyclophilin A; Cyclosporin A-binding protein; Rotamase A; SP18Cleaved into the following chain:Peptidyl-prolyl cis-trans isomerase A, N-terminally processed

PPIA

Ppia; Ppia; Cphn; CypA; CyP-18; 2700098C05; PPIase A

PPIA_MOUSE

Mouse

164

This assay has high sensitivity and excellent specificity for detection of Mouse PPIA. No significant cross-reactivity or interference between Mouse PPIA and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

For samples: For the quantitative determination of mouse cyclophilin A (CyPA) concentrations in serum, plasma, cell culture supernates, tissue homogenates. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CyPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CyPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CyPA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CyPA bound in the initial step. The color development is stopped and the intensity of the color is measured.

6679439

NP_032933.1

NM_008907.1

P17742

17,971 Da

Basigin Interactions Pathway (1001325); Cell Surface Interactions At The Vascular Wall Pathway (1001323); Hemostasis Pathway (1001295); Platelet Activation, Signaling And Aggregation Pathway (1001305); Platelet Degranulation Pathway (1001317); Response To Elevated Platelet Cytosolic Ca2+ Pathway (1001315)

Function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Catalytic activity: Peptidylproline (omega=180) = peptidylproline (omega=0). Enzyme regulation: Binds cyclosporin A (CsA). CsA mediates some of its effects via an inhibitory action on PPIase.

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800