catalog number :
MBS701266
products type :
ELISA Kit
products full name :
Rabbit diamine oxidase, DAO ELISA Kit
products short name :
D-amino-acid oxidase
products name syn :
Rabbit diamine oxidase (DAO) ELISA Kit; DAAO; DAMOX; MGC35381; OXDA; ; D-amino-acid oxidase
other names :
D-amino-acid oxidase; D-amino-acid oxidase; D-amino-acid oxidase; DAAO; DAMOX; D-amino acid oxidase
other gene names :
DAO; DAO; DAAO; DAMOX; DAO
uniprot entry name :
OXDA_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rabbit DAO. No significant cross-reactivity or interference between Rabbit DAO and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 1.25 U/ml-80 U/ml. Detection Wavelength: 450 nm. Sensitivity: 0.312 U/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DAO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DAO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAO is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAO bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075658.1
ncbi gb acc num :
NM_001082189.1
ncbi mol weight :
38,590 Da
ncbi pathways :
Arginine And Proline Metabolism Pathway 82957!!Arginine And Proline Metabolism Pathway 323!!D-Arginine And D-ornithine Metabolism Pathway 82972!!D-Arginine And D-ornithine Metabolism Pathway 341!!Glycine, Serine And Threonine Metabolism Pathway 82949!!Glycine, Serine And Threonine Metabolism Pathway 313!!Glyoxylate Metabolism Pathway 160990!!Metabolism Pathway 477135!!Metabolism Of Amino Acids And Derivatives Pathway 106169!!Peroxisome Pathway 131226
uniprot summary :
Function: Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids . Catalytic activity: A D-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2. Cofactor: FAD.