Mouse inducible nitric oxide synthase, iNOS ELISA Kit | MyBioSource MBS701257 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse inducible nitric oxide synthase, iNOS ELISA Kit

catalog :

MBS701257

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701257

products type :

ELISA Kit

products full name :

Mouse inducible nitric oxide synthase, iNOS ELISA Kit

products short name :

nitric oxide synthase 2, inducible

products name syn :

Mouse inducible nitric oxide synthase; iNOS ELISA KIT; HEP-NOS; INOS; NOS; NOS2A; NOS; type II; nitric oxide synthase 2A; nitric oxide synthase 2A (inducible; hepatocytes) ; nitric oxide synthase; macrophage; nitric oxide synthase 2; inducible

other names :

nitric oxide synthase, inducible; Nitric oxide synthase, inducible; nitric oxide synthase, inducible; MAC-NOS; NOS type II; inducible NOS; macrophage NOS; inducible NO synthase; inducible nitric oxide synthase; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2, inducible; Inducible NO synthase; Inducible NOS; iNOS; Macrophage NOS; MAC-NOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2

products gene name :

NOS2

other gene names :

Nos2; Nos2; iNOS; Nos-2; Nos2a; i-NOS; NOS-II; Inosl; Inducible NOS; iNOS; MAC-NOS

uniprot entry name :

NOS2_MOUSE

reactivity :

Mouse

sequence length :

1144

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse iNOS. No significant cross-reactivity or interference between mouse iNOS and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 7.8 IU/ml -500 IU/ml. Sensitivity: 1.95 IU/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for iNOS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any iNOS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for iNOS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of iNOS bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

6754872

ncbi acc num :

NP_035057.1

ncbi gb acc num :

NM_010927.3

uniprot acc num :

P29477

ncbi mol weight :

130,575 Da

ncbi pathways :

Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (83157); Arginine And Proline Metabolism Pathway (323); Calcium Signaling Pathway (83247); Calcium Signaling Pathway (459); Chagas Disease (American Trypanosomiasis) Pathway (147810); Chagas Disease (American Trypanosomiasis) Pathway (147795); Disease Pathway (970606); HIF-1 Signaling Pathway (695223)

ncbi summary :

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

uniprot summary :

Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.1 PublicationManual assertion based on experiment in:Ref.12. Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. Cofactor: Heme group. Enzyme regulation: Not stimulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity.

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage