Guinea Pig monocyte chemotactic protein 1, MCP1 ELISA Kit | MyBioSource MBS701253 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Guinea Pig monocyte chemotactic protein 1, MCP1 ELISA Kit

catalog :

MBS701253

quantity :

96 Strip Wells

price :

895 USD

product information

catalog number :

MBS701253

products type :

ELISA Kit

products full name :

Guinea Pig monocyte chemotactic protein 1, MCP1 ELISA Kit

products short name :

chemokine (C-C motif) ligand 2

products name syn :

Guinea pig monocyte chemotactic protein 1; MCP1 ELISA Kit; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; MGC9434; SCYA2; SMC-CF; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte secretory protein JE; small inducible cytokine; chemokine (C-C motif) ligand 2

other names :

C-C motif chemokine 2; C-C motif chemokine 2; C-C motif chemokine 2; small-inducible cytokine A2; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemoattractant protein 1; Monocyte chemotactic protein 1; MCP-1; Small-inducible cytokine A2

products gene name :

CCL2

other gene names :

Ccl2; CCL2; MCP1; MCP-1; SCYA2; MCP1; SCYA2; MCP-1

uniprot entry name :

CCL2_CAVPO

reactivity :

Guinea Pig

sequence length :

120

specificity :

This assay has high sensitivity and excellent specificity for detection of Guinea pig CCL2. No significant cross-reactivity or interference between Guinea pig CCL2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 23.5 pg/ml-1500 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 5.8 pg/ml

other info2 :

Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 2: chemokine. Protein Biological Process 3: Chemotaxis

products description :

Introduction: Chemokine (C-C motif) ligand 2 (MCP-1) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). MCP-1 recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing signal peptide of 23 amino acids and a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a molecular weight of approximately 13kDa. As with many other CC chemokines, MCP-1 is located on chromosome 17 in humans. The cell surface receptors that bind MCP-1 are CCR2 and CCR4. It is found at the site of tooth eruption and bone degradation. In the bone, MCP-1 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor kappaB (NFkappaB). In human osteoclasts, it has been shown that MCP-1 and RANTES (regulated on activation normal T cell expressed and secreted) are unregulated by RANKL (receptor activator of NFkappaB ligand). Both MCP-1 and RANTES were also shown to induce the formation of TRAP-positive, multinuclear cells from M-CSF-treated monocytes in the absence of RANKL, but produced osteoclasts that lacked cathepsin K expression and resorptive capacity. It is proposed that MCP-1 and RANTES act as autocrine loop in human osteoclast differentiation. MCP-1 causes the degranulation of basophils and mast cells, an effect potentiated by pre-treatment with IL-3 and other cytokines. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MCP-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MCP-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MCP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MCP-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

290543400

ncbi acc num :

NP_001166397.1

ncbi gb acc num :

NM_001172926.1

uniprot acc num :

Q08782

ncbi mol weight :

13,741 Da

ncbi pathways :

Activation Of Genes By ATF4 Pathway 530772!!Chagas Disease (American Trypanosomiasis) Pathway 147809!!Chagas Disease (American Trypanosomiasis) Pathway 147795!!Chemokine Receptors Bind Chemokines Pathway 106359!!Chemokine Signaling Pathway 99051!!Chemokine Signaling Pathway 96864!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Diabetes Pathways 105902

uniprot summary :

Function: Chemotactic factor that attracts monocytes, but not neutrophils.

size :

96 Strip Wells

price :

895 USD