ELISA/assay

Rat neutrophil gelatinase-associated lipocalin, NGAL ELISA Kit

MBS701246

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Rat neutrophil gelatinase-associated lipocalin, NGAL ELISA Kit

[lipocalin 2]

[Rat neutrophil gelatinase-associated lipocalin; NGAL ELISA Kit; 24p3; MSFI; NGAL; OTTHUMP00000022240; migration-stimulating factor inhibitor; neutrophil gelatinase-associated lipocalin; oncogene 24p3; siderocalin; lipocalin 2]

[neutrophil gelatinase-associated lipocalin; Neutrophil gelatinase-associated lipocalin; neutrophil gelatinase-associated lipocalin; p25; NGAL; lipocalin-2; siderocalin LCN2; lipocalin 2 (oncogene 24p3); alpha-2U globulin-related protein; alpha-2-microglobulin-related protein; lipocalin 2; Alpha-2-microglobulin-related protein; Alpha-2U globulin-related protein; Lipocalin-2; Siderocalin LCN2; p25]

[LCN2]

[Lcn2; Lcn2; Sip24; NGAL]

NGAL_RAT

Rat

198

This assay has high sensitivity and excellent specificity for detection of rat NGAL. No significant cross-reactivity or interference between rat NGAL and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: serum, plasma, urine, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: <0.078 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NGAL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NGAL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NGAL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NGAL bound in the initial step. The color development is stopped and the intensity of the color is measured.

18543345

NP_570097.1

NM_130741.1

P30152

22,476 Da

mouse homolog plays a role in IL3 withdrawal-induced apoptosis [RGD, Feb 2006]

Function: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

495

96-Strip-Wells

710

5x96-Strip-Wells

2705

10x96-Strip-Wells

5145