catalog number :
MBS701244
products type :
ELISA Kit
products full name :
Mouse Estradiol, E2 ELISA Kit
products short name :
Estradiol, E2
products name syn :
Mouse Estradiol; E2 ELISA Kit; Mouse Estradiol; E2
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse E2. No significant cross-reactivity or interference between mouse E2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Competitive. Detection Range: 25 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of mouse E2 is typically less than 25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest mouse E2 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for E2 and Horseradish Peroxidase (HRP) conjugated E2. The competitive inhibition reaction is launched between with HRP labeled E2 and unlabeled E2 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of E2 in the sample. The color development is stopped and the intensity of the color is measured.
size3 :
10x96-Strip-Wells