Rat CXC-chemokine receptor 4, CXCR4 ELISA Kit | MyBioSource MBS701239 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat CXC-chemokine receptor 4, CXCR4 ELISA Kit

catalog :

MBS701239

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701239

products type :

ELISA Kit

products full name :

Rat CXC-chemokine receptor 4, CXCR4 ELISA Kit

products short name :

chemokine (C-X-C motif) receptor 4

products name syn :

Rat CXC-chemokine receptor 4 (CXCR4) ELISA Kit; CD184; D2S201E; FB22; HM89; HSY3RR; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; WHIM; C-X-C chemokine receptor type 4; CD184 antigen; fusin; leukocyte-derived seven-transmembrane-domain receptor; lip; chemokine (C-X-C motif) receptor 4

other names :

C-X-C chemokine receptor type 4; C-X-C chemokine receptor type 4; C-X-C chemokine receptor type 4; LESTR; fusin; CXC-R4; CXCR-4; SDF-1 receptor; CXC chemokine receptor; chemokine receptor (LCR1); stromal cell-derived factor 1 receptor; leukocyte-derived seven transmembrane domain receptor; chemokine (C-X-C motif) receptor 4; Fusin; Leukocyte-derived seven transmembrane domain receptor; LESTR; Stromal cell-derived factor 1 receptor; SDF-1 receptor; CD_antigen: CD184

products gene name :

CXCR4

other gene names :

Cxcr4; Cxcr4; Cmkar4; CXC-R4; CXCR-4; LESTR; SDF-1 receptor

uniprot entry name :

CXCR4_RAT

reactivity :

Rat

sequence length :

349

specificity :

This assay has high sensitivity and excellent specificity for detection of rat CXCR4. No significant cross-reactivity or interference between rat CXCR4 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of rat CXCR4 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CXCR4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CXCR4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CXCR4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CXCR4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

2494971

ncbi acc num :

O08565.1

uniprot acc num :

O08565

ncbi mol weight :

39,334 Da

ncbi pathways :

Axon Guidance Pathway (83457); Axon Guidance Pathway (476); Chemokine Signaling Pathway (98755); Chemokine Signaling Pathway (96864); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Endocytosis Pathway (102267); Endocytosis Pathway (102181); GPCRs, Class A Rhodopsin-like Pathway (198500); IL-4 Signaling Pathway (198434)

ncbi summary :

G protein-coupled receptor for the chemokine stromal cell-derived factor-1; may have a role in brain development [RGD, Feb 2006]

uniprot summary :

Function: Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage