catalog number :
MBS701234
products type :
ELISA Kit
products full name :
Rat thrombin-antithrombin complex, TAT ELISA Kit
products short name :
thrombin-antithrombin complex, TAT
products name syn :
Rat thrombin-antithrombin complex; TAT ELISA Kit; thrombin-antithrombin complex; TAT
other names :
tat, partial; Protein Tat
uniprot entry name :
E2FMT9_9HIV1
specificity :
This assay has high sensitivity and excellent specificity for detection of rat TAT. No significant cross-reactivity or interference between rat TAT and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: 7.8 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAT bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
ADK70247.1
ncbi mol weight :
8,343 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells