ELISA/assay

Human mast cell tryptase, MCT ELISA Kit

MBS701200

48-Strip-Wells

565 USD

ELISA Kit

Human mast cell tryptase, MCT ELISA Kit

mast cell tryptase, MCT

Human mast cell tryptase; MCT ELISA Kit; mast cell tryptase; MCT

MCT; Monocarboxylate transporter 6; Monocarboxylate transporter 5; MCT 5; Solute carrier family 16 member 5

MCT

SLC16A5; MCT5; MCT6; MCT 6; MCT 5

MOT6_HUMAN

Human

505

This assay has high sensitivity and excellent specificity for detection of human MCT . No significant cross-reactivity or interference between human MCT and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml -100 ng/ml. Sensitivity: The minimum detectable dose of human MCT is typically less than 0.39 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MCT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCT bound in the initial step. The color development is stopped and the intensity of the color is measured.

2463630

AAC52013.1

O15375

54,994 Da

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

Function: Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150