product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
mouse Apelin ELISA Kit
catalog :
MBS701195
quantity :
96 Strip Wells
price :
850 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS701195
products type :
ELISA Kit
products full name :
mouse Apelin ELISA Kit
products short name :
apelin
products name syn :
mouse Apelin ELISA kit; XNPEP2; apelin; AGTRL1 ligand; peptide ligand for APJ receptor; apelin
other names :
apelin; Apelin; apelin; preproapelin; APJ endogenous ligand; apelin; APJ endogenous ligandCleaved into the following 4 chains:Apelin-36; Apelin-31; Apelin-28; Apelin-13
products gene name :
APLN
other gene names :
Apln; Apln; Apel; 6030430G11Rik; Apel
uniprot entry name :
APEL_MOUSE
reactivity :
Mouse
sequence length :
77
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse APLN. No significant cross-reactivity or interference between Mouse APLN and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 31.25 pg/ml-2000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 7.8 pg/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for apelin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any apelin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for apelin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of apelin bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
7304895
ncbi acc num :
NP_038940.1
ncbi gb acc num :
NM_013912.3
uniprot acc num :
Q9R0R4
ncbi mol weight :
8,658 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway 1000942!!G Alpha (i) Signalling Events Pathway 1000973!!GPCR Downstream Signaling Pathway 1000971!!GPCR Ligand Binding Pathway 1000941!!Peptide Ligand-binding Receptors Pathway 1000943!!Signal Transduction Pathway 970540!!Signaling By GPCR Pathway 971377
uniprot summary :
Function: Endogenous ligand for APJ. Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking. The oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior.
size :
96 Strip Wells
price :
850 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!