ELISA/assay

Rat Citrate synthase, CS ELISA Kit

MBS701194

96 Strip Wells

850 USD

ELISA Kit

Rat Citrate synthase, CS ELISA Kit

citrate synthase

Rat Citrate synthase; CS ELISA Kit; citrate synthase; mitochondrial; citrate synthase

citrate synthase, mitochondrial; Citrate synthase, mitochondrial; citrate synthase, mitochondrial; citrate (Si)-synthase; citrate synthase; Citrate (Si)-synthase

CS

Cs; Cs

CISY_RAT

Rat

466

This assay has high sensitivity and excellent specificity for detection of Rat CS. No significant cross-reactivity or interference between Rat CS and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates, cell lysates. Detection Range: 6.25 pg/ml-400 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 1.56 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 3: Tricarboxylic acid cycle

Introduction: The enzyme citrate synthase (E.C. 2.3.3.1) exists in nearly all living cells and stands as a pace-making enzyme in the first step of the Citric Acid Cycle (or Krebs Cycle). Citrate synthase is localized within eukaryotic cells in the mitochondrial matrix, but is encoded by nuclear DNA rather than mitochondrial. It is synthesized using cytoplasmic ribosomes, then transported into the mitochondrial matrix. Citrate synthase is commonly used as a quantitative enzyme marker for the presence of intact mitochondria. Citrate synthase catalyzes the condensation reaction of the two-carbon acetate residue from acetyl coenzyme A and a molecule of four-carbon oxaloacetate to form the six-carbon citrate. Oxaloacetate will be regenerated after the completion of one round of the Krebs Cycle. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

18543177

NP_570111.1

NM_130755.1

Q8VHF5

51,867 Da

2-Oxocarboxylic Acid Metabolism Pathway 716019!!2-Oxocarboxylic Acid Metabolism Pathway 717400!!Biosynthesis Of Amino Acids Pathway 791250!!Biosynthesis Of Amino Acids Pathway 795174!!Carbon Metabolism Pathway 816129!!Carbon Metabolism Pathway 817567!!Citrate Cycle (TCA Cycle) Pathway 83320!!Citrate Cycle (TCA Cycle) Pathway 288!!Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway 855837!!Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway 468202

human homolog is an enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A [RGD, Feb 2006]

96 Strip Wells

850 USD