ELISA/assay

Mouse Perforin/Pore-forming protein, PF/PFP ELISA Kit

MBS701191

48-Strip-Wells

565 USD

ELISA Kit

Mouse Perforin/Pore-forming protein, PF/PFP ELISA Kit

perforin 1 (pore forming protein)

Mouse Perforin/Pore-forming protein; PF/PFP ELISA Kit; RP11-710A11.3; FLH2; HPLH2; MGC65093; P1; PFN1; PFP; OTTHUMP00000019759; cytolysin; lymphocyte pore forming protein; perforin 1; perforin 1 (pore forming protein)

perforin-1; Perforin-1; perforin-1; P1; cytolysin; pore forming protein; lymphocyte pore-forming protein; perforin 1 (pore forming protein); Cytolysin; Lymphocyte pore-forming protein

PRF1

Prf1; Prf1; Pfn; Pfp; Prf-1; Pfp; P1

PERF_MOUSE

Mouse

554

This assay has high sensitivity and excellent specificity for detection of mouse PF/PFP. No significant cross-reactivity or interference between mouse PF/PFP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma and cell culture supernates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml -500 pg/ml. Sensitivity: The minimum detectable dose of mouse PF/PFP is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PF/PFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PF/PFP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PF/PFP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PF/PFP bound in the initial step. The color development is stopped and the intensity of the color is measured.

225735600

NP_035203.3

NM_011073.3

P10820

62,081 Da

Allograft Rejection Pathway (83316); Allograft Rejection Pathway (535); Apoptosis Pathway (198339); Autoimmune Thyroid Disease Pathway (83314); Autoimmune Thyroid Disease Pathway (533); Graft-versus-host Disease Pathway (83317); Graft-versus-host Disease Pathway (536); Natural Killer Cell Mediated Cytotoxicity Pathway (83276); Natural Killer Cell Mediated Cytotoxicity Pathway (490); Type I Diabetes Mellitus Pathway (83292)

Function: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.5 PublicationsManual assertion based on experiment in:Ref.3

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150