Human Alanine Aminotransferase, ALT/GPT ELISA Kit | MyBioSource MBS701171 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Alanine Aminotransferase, ALT/GPT ELISA Kit

catalog :

MBS701171

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701171

products type :

ELISA Kit

products full name :

Human Alanine Aminotransferase, ALT/GPT ELISA Kit

products short name :

glutamic-pyruvate transaminase (alanine aminotransferase)

products name syn :

Human Alanine Aminotransferase; ALT/GPT ELISA Kit; AAT1; ALT1; GPT1; glutamic pyruvate transaminase; glutamic-alanine transaminase 1; glutamic-pyruvate transaminase (alanine aminotransferase)

other names :

alanine aminotransferase 1; Alanine aminotransferase 1; alanine aminotransferase 1; GPT 1; glutamic-alanine transaminase 1; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1; glutamic-pyruvate transaminase (alanine aminotransferase); Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1

products gene name :

GPT

other gene names :

GPT; GPT; AAT1; ALT1; GPT1; AAT1; GPT1; ALT1; GPT 1

uniprot entry name :

ALAT1_HUMAN

reactivity :

Human

sequence length :

496

specificity :

This assay has high sensitivity and excellent specificity for detection of human ALT. No significant cross-reactivity or interference between human ALT and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 uIU/ml -1000 uIU/ml. Sensitivity: 3.9 uIU/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ALT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ALT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALT bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4885351

ncbi acc num :

NP_005300.1

ncbi gb acc num :

NM_005309.2

uniprot acc num :

P24298

ncbi mol weight :

54,637 Da

ncbi pathways :

2-Oxocarboxylic Acid Metabolism Pathway (714485); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (106173); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567)

ncbi summary :

This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]

uniprot summary :

Function: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles . Catalytic activity: L-alanine + 2-oxoglutarate = pyruvate + L-glutamate. Cofactor: Pyridoxal phosphate.

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage