catalog number :
MBS701164
products type :
ELISA Kit
products full name :
Bovine Insulin-like growth factor 1, IGF-1 ELISA Kit
products short name :
insulin-like growth factor 1 (somatomedin C)
products name syn :
Bovine Insulin-like growth factor 1; IGF-1 ELISA Kit; IGF1A; IGFI; OTTHUMP00000195084; insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C)
other names :
insulin-like growth factor I preproprotein; Insulin-like growth factor I; insulin-like growth factor I; somatomedin; prepro-IGF-I; insulin growth factor-1; insulin like growth factor 1; insulin-like growth factor-1; insulin-like growth factor I variant 1; insulin-like growth factor I (somatomedin C); class 1 insulin-like growth factor I preproprotein; insulin-like growth factor 1 (somatomedin C); Somatomedin
products gene name :
IGF1
other gene names :
IGF1; IGF1; IGF-1; IGF-I; IGF-I
uniprot entry name :
IGF1_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of bovine IGF-1. No significant cross-reactivity or interference between bovine IGF-1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 0.75 ng/ml -60 ng/ml. Sensitivity: 0.94 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for IGF-1 and Horseradish Peroxidase (HRP) conjugated IGF-1. The competitive inhibition reaction is launched between with HRP labeled IGF-1 and unlabeled IGF-1 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of IGF-1 in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001071296.1
ncbi gb acc num :
NM_001077828.1
ncbi mol weight :
17,066 Da
ncbi pathways :
AMPK Signaling Pathway (989258); AMPK Signaling Pathway (992181); Adipogenesis Pathway (920913); Aldosterone-regulated Sodium Reabsorption Pathway (130625); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Androgen Receptor Signaling Pathway (198553); Apoptosis Pathway (198572); Dilated Cardiomyopathy Pathway (121480); Dilated Cardiomyopathy Pathway (121285); Endochondral Ossification Pathway (920938)
uniprot summary :
Function: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation
size4 :
10x96-Strip-Wells