Human Cathepsin B, CTSB ELISA Kit | MyBioSource MBS701161 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Cathepsin B, CTSB ELISA Kit

catalog :

MBS701161

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS701161

products type :

ELISA Kit

products full name :

Human Cathepsin B, CTSB ELISA Kit

products short name :

[cathepsin B]

products name syn :

[Human cathepsin B (CTSB) ELISA kit; APPS; CPSB; APP secretase; amyloid precursor protein secretase; cathepsin B1; cysteine protease; preprocathepsin B; cathepsin B]

other names :

[cathepsin B preproprotein; Cathepsin B; cathepsin B; cathepsin B1; APP secretase; cysteine protease; amyloid precursor protein secretase; cathepsin B; APP secretase; APPS; Cathepsin B1Cleaved into the following 2 chains:Cathepsin B light chain; Cathepsin B heavy chain]

products gene name :

[CTSB]

other gene names :

[CTSB; CTSB; APPS; CPSB; CPSB; APPS]

uniprot entry name :

CATB_HUMAN

reactivity :

Human

sequence length :

339

specificity :

This assay has high sensitivity and excellent specificity for detection of human CTSB. No significant cross-reactivity or interference between human CTSB and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: Typically less than 0.078 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CTSB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTSB present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for CTSB is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTSB bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4503139

ncbi acc num :

NP_001899.1

ncbi gb acc num :

NM_001908.3

uniprot acc num :

P07858

ncbi mol weight :

37,822 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Degradation Pathway (730309); Collagen Formation Pathway (645288); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Immune System Pathway (106386); Innate Immune System Pathway (106387)

ncbi summary :

The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer disease, the most common cause of dementia. Overexpression of the encoded protein, which is a member of the peptidase C1 family, has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Catalytic activity: Hydrolysis of proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg- -Xaa bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides.

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

550

size3 :

96-Strip-Wells

price3 :

795

size4 :

5x96-Strip-Wells

price4 :

2890

size5 :

10x96-Strip-Wells

price5 :

5465

more info or order :

MyBioSource product webpage