ELISA/assay

Pig insulin-like growth factors-1, IGF-1 ELISA Kit

MBS701154

48-Strip-Wells

465 USD

ELISA Kit

Pig insulin-like growth factors-1, IGF-1 ELISA Kit

insulin-like growth factor 1 (somatomedin C)

Pig insulin-like growth factors-1; IGF-1 ELISA Kit; IGF1A; IGFI; OTTHUMP00000195084; insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C)

insulin-like growth factor I; Insulin-like growth factor I; insulin-like growth factor I; somatomedin C; Somatomedin

IGF1

IGF1; IGF1; IGF-I; Npt2B; IGF-I

IGF1_PIG

Pig

130

This assay has high sensitivity and excellent specificity for detection of pig IGF-1. No significant cross-reactivity or interference between pig IGF-1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 6.25 ng/ml -400 ng/ml. Sensitivity: 1.56 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGF-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGF-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGF-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGF-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

47523588

NP_999421.1

NM_214256.1

P16545

17,010 Da

AMPK Signaling Pathway (989938); AMPK Signaling Pathway (992181); Aldosterone-regulated Sodium Reabsorption Pathway (130624); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Dilated Cardiomyopathy Pathway (121477); Dilated Cardiomyopathy Pathway (121285); Focal Adhesion Pathway (84479); Focal Adhesion Pathway (478); FoxO Signaling Pathway (921549); Glioma Pathway (84517)

Function: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation

48-Strip-Wells

465 USD

96-Strip-Wells

655

5x96-Strip-Wells

2455

10x96-Strip-Wells

4595