ELISA/assay

Rat Factor-related Apoptosis, FAS ELISA Kit

MBS701147

48-Strip-Wells

510 USD

ELISA Kit

Rat Factor-related Apoptosis, FAS ELISA Kit

Fas (TNF receptor superfamily, member 6)

Rat Factor-related Apoptosis; FAS ELISA Kit; ALPS1A; APO-1; APT1; CD95; FAS1; FASTM; TNFRSF6; APO-1 cell surface antigen; CD95 antigen; Fas AMA; Fas antigen; apoptosis antigen 1; tumor necrosis factor receptor superfamily; member 6; Fas (TNF receptor superfamily; member 6)

tumor necrosis factor receptor superfamily member 6; Tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily member 6; CD95 antigen; Fas receptor; apo-1 antigen; FASLG receptor; Fas antigen (ATP1); apoptosis-mediating surface antigen FAS; Fas (TNF receptor superfamily, member 6); Tumor necrosis factor receptor superfamily, member 6; Fas cell surface death receptor; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD_antigen: CD95

FAS

Fas; Fas; Tnfrsf6; Apt1; Tnfrsf6

TNR6_RAT

Rat

324

This assay has high sensitivity and excellent specificity for detection of rat FAS. No significant cross-reactivity or interference between rat FAS and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.78 ng/ml -50 ng/ml. Sensitivity: The minimum detectable dose of rat FAS is typically less than 0.195 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FAS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FAS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FAS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FAS bound in the initial step. The color development is stopped and the intensity of the color is measured.

59624977

NP_631933.2

NM_139194.2

Q63199

36,835 Da

Adipogenesis Pathway (198479); African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83511); Allograft Rejection Pathway (535); Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Apoptosis Pathway (219785); Apoptosis Pathway (83452); Apoptosis Pathway (470)

Tnfsf6/Fasl receptor [RGD, Feb 2006]

Function: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800