ELISA/assay

Rat 3-hydroxy-3-methylglutaryl-Coenzyme A synthase, HMGCS ELISA Kit

MBS701144

48-Strip-Wells

565 USD

ELISA Kit

Rat 3-hydroxy-3-methylglutaryl-Coenzyme A synthase, HMGCS ELISA Kit

3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble)

Rat 3-hydroxy-3-methylglutaryl-Coenzyme A synthase; HMGCS ELISA Kit; HMGCS; MGC90332; 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase; hydroxymethylglutaryl-CoA synthase 1; 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble)

hydroxymethylglutaryl-CoA synthase, cytoplasmic; Hydroxymethylglutaryl-CoA synthase, cytoplasmic; hydroxymethylglutaryl-CoA synthase, cytoplasmic; HMG-CoA synthase; hydroxymethylglutaryl-CoA synthase 1; 3-hydroxy-3-methylglutaryl coenzyme A synthase; 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble); 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble); 3-hydroxy-3-methylglutaryl coenzyme A synthase

HMGCS1

Hmgcs1; Hmgcs1; Hmgcs; HMG-CoA synthase

HMCS1_RAT

Rat

520

This assay has high sensitivity and excellent specificity for detection of rat HMGCS. No significant cross-reactivity or interference between rat HMGCS and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of rat HMGCS is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HMGCS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HMGCS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HMGCS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HMGCS bound in the initial step. The color development is stopped and the intensity of the color is measured.

8393538

NP_058964.1

NM_017268.1

P17425

57,434 Da

Activation Of Gene Expression By SREBF (SREBP) Pathway (1010720); Butanoate Metabolism Pathway (83399); Butanoate Metabolism Pathway (391); C5 Isoprenoid Biosynthesis, Mevalonate Pathway (434703); C5 Isoprenoid Biosynthesis, Mevalonate Pathway (468288); Cholesterol Biosynthesis Pathway (198457); Cholesterol Biosynthesis Pathway (1010718); Cholesterol Metabolism Pathway (198518); Cholesterol Metabolism Pathway (198522); Endochondral Ossification Pathway (219771)

catalyzes the conversion of (S)-3-hydroxy-3-methylglutaryl-CoA and CoA to acetyl-CoA, acetoacetyl-CoA, and H2O [RGD, Feb 2006]

Function: This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. Catalytic activity: Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150