catalog number :
MBS701139
products type :
ELISA Kit
products full name :
Mouse Somatostatin, SS ELISA Kit
products short name :
somatostatin
products name syn :
Mouse Somatostatin; SS ELISA Kit; SMST; somatostatin-14; somatostatin-28; somatostatin
other names :
somatostatin; Somatostatin; somatostatin; preprosomatostatin; somatostatin
other gene names :
Sst; Sst; SS; SOM; SRIF; Smst; Smst
uniprot entry name :
SMS_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse SS. No significant cross-reactivity or interference between mouse SS and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 4. 7 pg/ml -300 pg/ml. Sensitivity: The minimum detectable dose of mouse SS is typically less than 1.1 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SS bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_033241.1
ncbi gb acc num :
NM_009215.1
ncbi mol weight :
12,746 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway (1000942); G Alpha (i) Signalling Events Pathway (1000973); GPCR Downstream Signaling Pathway (1000971); GPCR Ligand Binding Pathway (1000941); Gastric Acid Secretion Pathway (154411); Gastric Acid Secretion Pathway (154383); Peptide Ligand-binding Receptors Pathway (1000943); Signal Transduction Pathway (970540); Signaling By GPCR Pathway (971377)
uniprot summary :
Function: Somatostatin inhibits the release of somatotropin.
size4 :
10x96-Strip-Wells