catalog number :
MBS701134
products type :
ELISA Kit
products full name :
Rat Vitamin B12, VB12 ELISA Kit
products short name :
Vitamin B12, VB12
products name syn :
Rat Vitamin B12; VB12 ELISA Kit; Rat Vitamin B12; VB12
products gene name :
VB12
specificity :
This assay has high sensitivity and excellent specificity for detection of rat VB12. No significant cross-reactivity or interference between rat VB12 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Competitive. Detection Range: 0.312 pg/ml -20 pg/ml. Sensitivity: The minimum detectable dose of rat VB12 is typically less than 0.299 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest rat VB12 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for VB12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated VB12. A competitive inhibition reaction is launched between VB12 (Standards or samples) and biotin-conjugated VB12 with the pre-coated VB12 antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of VB12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
size3 :
10x96-Strip-Wells