product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Vitamin B12, VB12 ELISA Kit
catalog :
MBS701134
quantity :
96-Strip-Wells
price :
795 USD
more info or order :
product information
catalog number :
MBS701134
products type :
ELISA Kit
products full name :
Rat Vitamin B12, VB12 ELISA Kit
products short name :
Vitamin B12, VB12
products name syn :
Rat Vitamin B12; VB12 ELISA Kit; Rat Vitamin B12; VB12
products gene name :
VB12
reactivity :
Rat
specificity :
This assay has high sensitivity and excellent specificity for detection of rat VB12. No significant cross-reactivity or interference between rat VB12 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Competitive. Detection Range: 0.312 pg/ml -20 pg/ml. Sensitivity: The minimum detectable dose of rat VB12 is typically less than 0.299 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest rat VB12 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for VB12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated VB12. A competitive inhibition reaction is launched between VB12 (Standards or samples) and biotin-conjugated VB12 with the pre-coated VB12 antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of VB12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
size1 :
96-Strip-Wells
price1 :
795 USD
size2 :
5x96-Strip-Wells
price2 :
2435
size3 :
10x96-Strip-Wells
price3 :
4555
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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