catalog number :
MBS701131
products type :
ELISA Kit
products full name :
Rat inositol 1, 4, 5, -trisphosphate, IP3 ELISA Kit
products short name :
inositol 1, 4, 5, -trisphosphate (IP3)
products name syn :
Rat inositol 1; 4; 5; -trisphosphate (IP3) ELISA kit; inositol 1; 4; 5; -trisphosphate (IP3)
other names :
Ip3; Ip3; Ip3
uniprot entry name :
Q38387_BPK3
specificity :
This assay has high sensitivity and excellent specificity for detection of rat IP3. No significant cross-reactivity or interference between rat IP3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell culture supernates and cell lysates. Assay Type: Quantitative Competitive. Sensitivity: Typically less than 2.5 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for IP3 and Horseradish Peroxidase (HRP) conjugated IP3. The competitive inhibition reaction is launched between with HRP labeled IP3 and unlabeled IP3 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of IP3 in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
CAA84450.1
ncbi mol weight :
21,705 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells