ELISA/assay

Rat Amylin ELISA Kit

MBS701130

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Rat Amylin ELISA Kit

[Amylin]

[Rat Amylin ELISA Kit; Amylin]

[amylin, partial; Islet amyloid polypeptide; islet amyloid polypeptide; amylin; insulinoma amyloid peptide; diabetes-associated peptide; Islet amyloid polypeptide (diabetes-associated peptide; amylin); islet amyloid polypeptide; Amylin; Diabetes-associated peptide; DAP; Insulinoma amyloid peptide]

[Amylin]

[IAPP; IAPP; DAP; IAP; DAP]

IAPP_HUMAN

Rat

62

This assay has high sensitivity and excellent specificity for detection of human AMH. No significant cross-reactivity or interference between human AMH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: 15.6 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AMH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AMH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMH bound in the initial step. The color development is stopped and the intensity of the color is measured.

457131

AAA35524.1

P10997

9,806 Da

Amyloids Pathway (366238); Calcitonin-like Ligand Receptors Pathway (106379); Class B/2 (Secretin Family Receptors) Pathway (106378); Developmental Biology Pathway (477129); Disease Pathway (530764); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Integrated Pancreatic Cancer Pathway (711360); Maturity Onset Diabetes Of The Young Pathway (83096)

Islet, or insulinoma, amyloid polypeptide is commonly found in pancreatic islets of patients suffering diabetes mellitus type II, or harboring an insulinoma. While the association of amylin with the development of type II diabetes has been known for some time, a direct causative role for amylin has been harder to establish. Studies suggest that amylin, like the related beta-amyloid (Abeta) associated with Alzheimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the development of type II diabetes. [provided by RefSeq, Apr 2011]

Function: Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

590

96-Strip-Wells

855

5x96-Strip-Wells

3110

10x96-Strip-Wells

5890