catalog number :
MBS701129
products type :
ELISA Kit
products full name :
Mouse tissue inhibitors of metalloproteinase 2, TIMP-2 ELISA Kit
products short name :
TIMP metallopeptidase inhibitor 2
products name syn :
Mouse tissue inhibitors of metalloproteinase 2; TIMP-2 ELISA kit; CSC-21K; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinases 2; TIMP metallopeptidase inhibitor 2
other names :
Metalloproteinase inhibitor 2; Metalloproteinase inhibitor 2; metalloproteinase inhibitor 2; tissue inhibitor of metalloproteinases 2; tissue inhibitor of metalloproteinase 2; Tissue inhibitor of metalloproteinases 2; TIMP-2
products gene name :
TIMP2
other gene names :
Timp2; Timp2; Timp-2; D11Bwg1104e; Timp-2; TIMP-2
uniprot entry name :
TIMP2_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse TIMP-2. No significant cross-reactivity or interference between mouse TIMP-2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 125 pg/ml -8000 pg/ml. Sensitivity: The minimum detectable dose of mouse TIMP-2 is typically less than 31.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TIMP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIMP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
24,328 Da
ncbi pathways :
Activation Of Matrix Metalloproteinases Pathway (1001029); Degradation Of The Extracellular Matrix Pathway (1001028); Extracellular Matrix Organization Pathway (1001015); Matrix Metalloproteinases Pathway (198370)
uniprot summary :
Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
size4 :
10x96-Strip-Wells