Mouse Peroxisome Proliferator-activated receptor gamma, PPAR-gamma ELISA Kit | MyBioSource MBS701124 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Peroxisome Proliferator-activated receptor gamma, PPAR-gamma ELISA Kit

catalog :

MBS701124

quantity :

48-Strip-Wells

price :

590 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701124

products type :

ELISA Kit

products full name :

Mouse Peroxisome Proliferator-activated receptor gamma, PPAR-gamma ELISA Kit

products short name :

peroxisome proliferator-activated receptor gamma

products name syn :

Mouse Peroxisome Proliferator-activated receptor gamma; PPAR-gamma ELISA Kit; CIMT1; GLM1; NR1C3; PPARG1; PPARG2; PPARgamma; OTTHUMP00000185030; OTTHUMP00000185033; PPAR gamma; nuclear receptor subfamily 1 group C member 3; peroxisome proliferative activated receptor gamma; peroxi; peroxisome proliferator-activated receptor gamma

other names :

peroxisome proliferator-activated receptor gamma isoform 1; Peroxisome proliferator-activated receptor gamma; peroxisome proliferator-activated receptor gamma; nuclear receptor subfamily 1 group C member 3; peroxisome proliferator activated receptor gamma 2; peroxisome proliferator activated receptor gamma 4; peroxisome proliferator activated receptor gamma; Nuclear receptor subfamily 1 group C member 3

products gene name :

PPARG

other gene names :

Pparg; Pparg; Nr1c3; PPARgamma; PPAR-gamma; PPARgamma2; PPAR-gamma2; Nr1c3; PPAR-gamma

uniprot entry name :

PPARG_MOUSE

reactivity :

Mouse

sequence length :

475

specificity :

This assay has high sensitivity and excellent specificity for detection of Mouse PPARG. No significant cross-reactivity or interference between Mouse PPARG and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Detection Range: 0.625 pg/ml-40 pg/ml. Sensitivity: 0.156 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transcription/Transcription regulation. Protein Biological Process 3: Transcription

products description :

Introduction: Peroxisome proliferators are non genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. The PPAR gamma 2 isoform appears to be induced very early in the differentiation of several cultured adipocyte cell lines, and has been suggested to be a dominant regulator of the murine P2 (aP2) gene which encodes an intracellular lipid binding protein which is expressed only in adipose cells. PPAR gamma 2, like several other nuclear hormone receptors, heterodimerizes with RXR alpha. Defects in PPAR gamma can lead to type 2 insulin-resistant diabetes and hyptertension and could play a role in the genetic predisposition to obesity. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPAR-gamma. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PPAR-gamma and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PPAR-gamma, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PPAR-gamma in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

187960105

ncbi acc num :

NP_001120802.1

ncbi gb acc num :

NM_001127330.1

uniprot acc num :

P37238

ncbi mol weight :

57,598 Da

ncbi pathways :

AMPK Signaling Pathway (989651); AMPK Signaling Pathway (992181); Adipogenesis Pathway (198299); Developmental Biology Pathway (970813); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (970709); Gene Expression Pathway (971427); Generic Transcription Pathway (1000465); Huntington's Disease Pathway (83297); Huntington's Disease Pathway (512); Metabolism Pathway (971233)

ncbi summary :

This gene encodes a nuclear receptor protein belonging to the peroxisome proliferator-activated receptor (Ppar) family. The encoded protein is a ligand-activated transcription factor that is involved in the regulation of adipocyte differentiation and glucose homeostasis. The encoded protein forms a heterodimer with retinoid X receptors and binds to DNA motifs termed "peroxisome proliferator response elements" to either activate or inhibit gene expression. Mice lacking the encoded protein die at an embryonic stage due to severe defects in placental vascularization. When the embryos lacking this gene are supplemented with healthy placentas, the mutants survive to term, but succumb to lipodystrophy and multiple hemorrhages. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

uniprot summary :

Function: Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses.4 PublicationsManual assertion based on experiment in:Ref.11. Enzyme regulation: PDPK1 activates its transcriptional activity independently of its kinase activity

size1 :

48-Strip-Wells

price1 :

590 USD

size2 :

96-Strip-Wells

price2 :

850

size3 :

5x96-Strip-Wells

price3 :

2880

size4 :

10x96-Strip-Wells

price4 :

5440

more info or order :

MyBioSource product webpage