catalog number :
MBS701120
products type :
ELISA Kit
products full name :
rabbit Interleukin 4, IL-4 ELISA Kit
products short name :
interleukin 4
products name syn :
rabbit Interleukin 4; IL-4 ELISA Kit; BCGF-1; BCGF1; BSF1; IL-4; MGC79402; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; interleukin 4
other names :
interleukin-4 isoform 1; Interleukin-4; interleukin-4; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell stimulatory factor 1; BSF-1; Lymphocyte stimulatory factor 1
other gene names :
IL4; IL4; IL2; BSF-1; IL-4; BSF-1
uniprot entry name :
IL4_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rabbit IL4. No significant cross-reactivity or interference between Rabbit IL4 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 pg/ml-40 pg/ml. Sensitivity: 0.156 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: B-cell activation
products description :
For Samples: For the quantitative determination of rabbit interleukin 4 (IL-4) concentrations in serum, plasma, tissue homogenates. Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001156649.1
ncbi gb acc num :
NM_001163177.1
ncbi mol weight :
16,562 Da
ncbi pathways :
Allograft Rejection Pathway (84530); Allograft Rejection Pathway (535); Asthma Pathway (84527); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (84528); Autoimmune Thyroid Disease Pathway (533); Cytokine-cytokine Receptor Interaction Pathway (84463); Cytokine-cytokine Receptor Interaction Pathway (460); Fc Epsilon RI Signaling Pathway (84494); Fc Epsilon RI Signaling Pathway (493)
uniprot summary :
Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes
size4 :
10x96-Strip-Wells