Human lumican, LUM ELISA Kit | MyBioSource MBS701117 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human lumican, LUM ELISA Kit

catalog :

MBS701117

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

citations: 1

Reference
Kang Y, Roife D, Lee Y, Lv H, Suzuki R, Ling J, et al. Transforming Growth Factor-? Limits Secretion of Lumican by Activated Stellate Cells within Primary Pancreatic Adenocarcinoma Tumors. Clin Cancer Res. 2016;22:4934-4946 pubmed

product information

catalog number :

MBS701117

products type :

ELISA Kit

products full name :

Human lumican, LUM ELISA Kit

products short name :

lumican

products name syn :

Human lumican; LUM ELISA Kit; LDC; SLRR2D; lumican proteoglycan; lumican

other names :

lumican; Lumican; lumican; KSPG lumican; lumican proteoglycan; keratan sulfate proteoglycan lumican; lumican; Keratan sulfate proteoglycan lumican; KSPG lumican

products gene name :

LUM

other gene names :

LUM; LUM; LDC; SLRR2D; LDC; SLRR2D; KSPG lumican

uniprot entry name :

LUM_HUMAN

reactivity :

Human

sequence length :

338

specificity :

This assay has high sensitivity and excellent specificity for detection of human LUM. No significant cross-reactivity or interference between human LUM and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, urine, urine, urine, urine, urine, urine, cell culture supernates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of human LUM is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LUM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LUM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LUM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LUM bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4505047

ncbi acc num :

NP_002336.1

ncbi gb acc num :

NM_002345.3

uniprot acc num :

P51884

ncbi mol weight :

38,429 Da

ncbi pathways :

Disease Pathway (530764); ECM Proteoglycans Pathway (833812); Extracellular Matrix Organization Pathway (576262); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Metabolism Pathway (645297); Integrin Cell Surface Interactions Pathway (106110); Keratan Sulfate Biosynthesis Pathway (645302); Keratan Sulfate Degradation Pathway (645303); Keratan Sulfate/keratin Metabolism Pathway (645301); MPS I - Hurler Syndrome Pathway (685537)

ncbi summary :

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]

uniprot summary :

LUM: a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12q21.33. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; lysosomal lumen; fibrillar collagen; Golgi lumen; extracellular region. Molecular Function: collagen binding; protein binding; extracellular matrix structural constituent. Biological Process: keratan sulfate metabolic process; extracellular matrix organization and biogenesis; collagen fibril organization; glycosaminoglycan metabolic process; visual perception; cartilage development; carbohydrate metabolic process; keratan sulfate biosynthetic process; pathogenesis; positive regulation of transcription from RNA polymerase II promoter; keratan sulfate catabolic process; response to organic cyclic substance

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage