catalog number :
MBS701111
products type :
ELISA Kit
products full name :
Mouse hepatocyte growth factor, HGF ELISA Kit
products short name :
hepatocyte growth factor (hepapoietin A; scatter factor)
products name syn :
Mouse hepatocyte growth factor; HGF ELISA kit; DFNB39; F-TCF; HGFB; HPTA; SF; OTTHUMP00000206710; fibroblast-derived tumor cytotoxic factor; hepatocyte growth factor; lung fibroblast-derived mitogen; hepatocyte growth factor (hepapoietin A; scatter factor)
other names :
hepatocyte growth factor isoform 1 preproprotein; Hepatocyte growth factor; hepatocyte growth factor; scatter factor; hepatopoietin-A; hepatocyte growth factor; Hepatopoietin-A; Scatter factor; SF
other gene names :
Hgf; Hgf; SF; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik; SF
uniprot entry name :
HGF_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse HGF. No significant cross-reactivity or interference between mouse HGF and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of mouse HGF is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001276387.1
ncbi gb acc num :
NM_001289458.1
ncbi mol weight :
82,945 Da
ncbi pathways :
Cytokine Signaling In Immune System Pathway (970814); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Focal Adhesion Pathway (198353); Focal Adhesion Pathway (83264); Focal Adhesion Pathway (478); Hemostasis Pathway (1001295); Immune System Pathway (971216); Interleukin-7 Signaling Pathway (1001293); Malaria Pathway (152666)
uniprot summary :
Function: Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.1 PublicationManual assertion based on experiment in:Ref.13
size4 :
10x96-Strip-Wells