ELISA/assay

Horse Tumor necrosis factor alpha, TNF-alpha ELISA Kit

MBS701096

96 Strip Wells

915 USD

ELISA Kit

Horse Tumor necrosis factor alpha, TNF-alpha ELISA Kit

tumor necrosis factor (TNF superfamily, member 2)

Horse Tumor necrosis factor alpha; TNF-alpha ELISA Kit; DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein; TNF superfamily; member 2; TNF; macrophage-derived; TNF; monocyte-derived; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily; member 2)

tumor necrosis factor; Tumor necrosis factor; tumor necrosis factor; TNF-alpha; cachectin; tumor necrosis factor-alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

TNF

TNF; TNF; TNFA; TNF-a; TNFSF2; TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2

TNFA_HORSE

Horse

234

This assay has high sensitivity and excellent specificity for detection of Horse TNF. No significant cross-reactivity or interference between Horse TNF and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 15.6 pg/ml-1000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 3.9 pg/ml

Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TNF-alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TNF-alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TNF-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

126352375

NP_001075288.1

NM_001081819.1

P29553

25,469 Da

Adipocytokine Signaling Pathway 90046!!Adipocytokine Signaling Pathway 505!!African Trypanosomiasis Pathway 194408!!African Trypanosomiasis Pathway 194323!!Allograft Rejection Pathway 90072!!Allograft Rejection Pathway 535!!Alzheimer's Disease Pathway 90050!!Alzheimer's Disease Pathway 509!!Amoebiasis Pathway 167381!!Amoebiasis Pathway 167191

Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

96 Strip Wells

915 USD