ELISA/assay

Human lipoprotein lipase, LPL ELISA Kit

MBS701093

48-Strip-Wells

565 USD

ELISA Kit

Human lipoprotein lipase, LPL ELISA Kit

lipoprotein lipase

Human lipoprotein lipase (LPL) ELISA Kit; HDLCQ11; LIPD; ; lipoprotein lipase

lipoprotein lipase; Lipoprotein lipase; lipoprotein lipase; lipoprotein lipase

LPL

LPL; LPL; LIPD; HDLCQ11; LIPD; LPL

LIPL_HUMAN

Human

475

This assay has high sensitivity and excellent specificity for detection of human LPL. No significant cross-reactivity or interference between human LPL and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of human LPL is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LPL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LPL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPL bound in the initial step. The color development is stopped and the intensity of the color is measured.

4557727

NP_000228.1

NM_000237.2

P06858

53,162 Da

Adipogenesis Pathway (198832); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Chylomicron-mediated Lipid Transport Pathway (106157); Developmental Biology Pathway (477129); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Fatty Acid Beta Oxidation Pathway (198865); Glycerolipid Metabolism Pathway (82986)

LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq, Jul 2008]

Function: The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium . Catalytic activity: Triacylglycerol + H2O = diacylglycerol + a carboxylate.1 PublicationManual assertion based on experiment in:Ref.11

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150