product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
human soluble cluster of differentiation 28, sCD28 ELISA Kit
catalog :
MBS701080
quantity :
48-Strip-Wells
price :
450 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS701080
products type :
ELISA Kit
products full name :
human soluble cluster of differentiation 28, sCD28 ELISA Kit
products short name :
CD28 molecule
products name syn :
human soluble cluster of differentiation 28; sCD28 ELISA Kit; MGC138290; Tp44; CD28 antigen; CD28 antigen (Tp44) ; T-cell-specific surface glycoprotein; CD28 molecule
other names :
T-cell-specific surface glycoprotein CD28 isoform 2; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28; CD28 antigen; CD28 molecule; TP44; CD_antigen: CD28
products gene name :
CD28
other gene names :
CD28; CD28; Tp44
uniprot entry name :
CD28_HUMAN
reactivity :
Human
sequence length :
123
specificity :
This assay has high sensitivity and excellent specificity for detection of human sCD28. No significant cross-reactivity or interference between human sCD28 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of human sCD28 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sCD28 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD28 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sCD28 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD28 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
340545508
ncbi acc num :
NP_001230006.1
ncbi gb acc num :
NM_001243077.1
uniprot acc num :
P10747
ncbi mol weight :
25,066 Da
ncbi pathways :
Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); CD28 Co-stimulation Pathway (119553); CD28 Dependent PI3K/Akt Signaling Pathway (119554); CD28 Dependent Vav1 Pathway (119555); Cell Adhesion Molecules (CAMs) Pathway (83069)
ncbi summary :
The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
uniprot summary :
Function: Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
size1 :
48-Strip-Wells
price1 :
450 USD
size2 :
96-Strip-Wells
price2 :
640
size3 :
5x96-Strip-Wells
price3 :
2435
size4 :
10x96-Strip-Wells
price4 :
4555
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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