ELISA/assay

Human vitamin K-dependent protein Z, PROZ ELISA Kit

MBS701077

48-Strip-Wells

565 USD

ELISA Kit

Human vitamin K-dependent protein Z, PROZ ELISA Kit

vitamin K-dependent protein Z

Human vitamin K-dependent protein Z (PROZ) ELISA kit; PZ; protein Z; vitamin K-dependent plasma glycoprotein; vitamin K-dependent protein Z

vitamin K-dependent protein Z isoform 1; Vitamin K-dependent protein Z; vitamin K-dependent protein Z; protein Z, vitamin K-dependent plasma glycoprotein

PROZ

PROZ; PROZ; PZ

PROZ_HUMAN

Human

422

This assay has high sensitivity and excellent specificity for detection of Human PROZ. No significant cross-reactivity or interference between Human PROZ and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 125 ng/ml-8000 ng/ml. Sensitivity: 31.25 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Blood Coagulation. Protein Biological Process 3: Blood coagulation

For samples: For the quantitative determination of human protein Z concentrations in serum, plasma, tissue homogenates. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for protein Z has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any protein Z present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for protein Z is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of protein Z bound in the initial step. The color development is stopped and the intensity of the color is measured.

371502106

NP_001243063.1

NM_001256134.1

P22891

44,744 Da

Gamma-carboxylation Of Protein Precursors Pathway (106233); Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway (106232); Metabolism Of Proteins Pathway (106230); PTM: Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway (106231); Post-translational Protein Modification Pathway (161012); Removal Of Aminoterminal Propeptides From Gamma-carboxylated Proteins Pathway (106235); Transport Of Gamma-carboxylated Protein Precursors From The Endoplasmic Reticulum To The Golgi Apparatus Pathway (106234)

This gene encodes a liver vitamin K-dependent glycoprotein that is synthesized in the liver and secreted into the plasma. The encoded protein plays a role in regulating blood coagulation by complexing with protein Z-dependent protease inhibitor to directly inhibit activated factor X at the phospholipid surface. Deficiencies in this protein are associated with an increased risk of ischemic arterial diseases and fetal loss. Mutations in this gene are the cause of protein Z deficiency. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Function: Appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. Inhibits activity of the coagulation protease factor Xa in the presence of SERPINA10, calcium and phospholipids.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150