catalog number :
MBS701070
products type :
ELISA Kit
products full name :
Rat Tumor necrosis factor soluble receptor II, TNFsR-II ELISA Kit
products short name :
Tumor necrosis factor soluble receptor II, TNFsR-
products name syn :
Rat Tumor necrosis factor soluble receptor II; TNFsR-II ELISA KIT; CD120b; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR1B; TNFR2; TNFR80; p75; p75TNFR; p75 TNF receptor; soluble TNFR1B variant 1; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor soluble receptor II; TNFsR-
other names :
tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily member 1B; p75; TNF-R2; TNF-RII; TNFR-II; p80 TNF-alpha receptor; tumor necrosis factor receptor 2; tumor necrosis factor receptor type II; tumor necrosis factor receptor superfamily, member 1b; Tumor necrosis factor receptor 2; TNF-R2; Tumor necrosis factor receptor type II; TNF-RII; TNFR-II; p75; p80 TNF-alpha receptor; CD_antigen: CD120b
products gene name :
TNFsR-
other gene names :
Tnfrsf1b; Tnfrsf1b; Tnfr2; Tnfr2; TNF-R2; TNF-RII; TNFR-II
uniprot entry name :
TNR1B_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat TNFsR-. No significant cross-reactivity or interference between Rat TNFsR- and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: 15.6 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TNFsR-II has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFsR-II present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFsR-II is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFsR-II bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_569110.1
ncbi gb acc num :
NM_130426.4
ncbi mol weight :
50,148 Da
ncbi pathways :
Adipocytokine Signaling Pathway (83485); Adipocytokine Signaling Pathway (505); Amyotrophic Lateral Sclerosis (ALS) Pathway (83491); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (219785); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); IL-3 Signaling Pathway (198504); Inflammatory Response Pathway (198472); TNF Signaling Pathway (812267)
ncbi summary :
mediates brain uptake of tumor necrosis factor (TNF)-alpha from the circulation [RGD, Feb 2006]
uniprot summary :
Function: Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2
size4 :
10x96-Strip-Wells