ELISA/assay

Mouse peroxisome proliferators activator receptors alpha, PPAR-alpha ELISA Kit

MBS701064

96 Strip Wells

575 USD

ELISA Kit

Mouse peroxisome proliferators activator receptors alpha, PPAR-alpha ELISA Kit

peroxisome proliferator-activated receptor alpha

Mouse peroxisome proliferators activator receptors alpha; PPAR-alpha ELISA Kit; MGC2237; MGC2452; NR1C1; PPAR; PPARalpha; hPPAR; OTTHUMP00000197740; OTTHUMP00000197741; peroxisome proliferative activated receptor; alpha; peroxisome proliferator-activated receptor alpha

peroxisome proliferator-activated receptor alpha; Peroxisome proliferator-activated receptor alpha; peroxisome proliferator-activated receptor alpha; nuclear receptor subfamily 1 group C member 1; peroxisome proliferator activated receptor alpha; Nuclear receptor subfamily 1 group C member 1

PPARA

Ppara; Ppara; Ppar; Nr1c1; AW742785; PPARalpha; PPAR-alpha; 4933429D07Rik; Nr1c1; Ppar; PPAR-alpha

PPARA_MOUSE

Mouse

468

This assay has high sensitivity and excellent specificity for detection of Mouse PPARA. No significant cross-reactivity or interference between Mouse PPARA and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm

Sample Volume: 50-100ul. Protein Biological Process 1: Transcription/Transcription regulation. Protein Biological Process 3: Transcription

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPAR-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotinconjugated antibody preparation specific for PPAR-alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PPAR-alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PPAR-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

164663880

NP_001106889.1

NM_001113418.1

P23204

52,347 Da

Activation Of Gene Expression By SREBF (SREBP) Pathway 1001054!!Adipocytokine Signaling Pathway 83290!!Adipocytokine Signaling Pathway 505!!Adipogenesis Pathway 198299!!BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway 1000462!!Bmal1:Clock,Npas2 Activates Circadian Gene Expression Pathway 966898!!Circadian Clock Pathway 966897!!Circadian Clock Pathway 1000461!!Developmental Biology Pathway 970813!!Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway 970709

Function: Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.3 PublicationsManual assertion based on experiment in:Ref.8

96 Strip Wells

575 USD