ELISA/assay

Dog Endothelial nitric oxide synthase, eNOS ELISA Kit

MBS701030

96 Strip Wells

915 USD

ELISA Kit

Dog Endothelial nitric oxide synthase, eNOS ELISA Kit

nitric oxide synthase 3 (endothelial cell)

Dog Endothelial nitric oxide synthase; eNOS ELISA Kit; ECNOS; eNOS; nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell)

Nitric oxide synthase, endothelial; Nitric oxide synthase, endothelial; nitric oxide synthase, endothelial; cNOS; eNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII

NOS3

NOS3; NOS3; NOS; NOS; cNOS; eNOS; NOSIII

NOS3_CANFA

Dog

1205

This assay has high sensitivity and excellent specificity for detection of dog eNOS. No significant cross-reactivity or interference between dog eNOS and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of dog endothelial nitric oxide synthase (eNOS) concentrations in serum, plasma, tissue homogenates, cell lysates. Detection Range: 15.6 U/ml -1000U/ml.

Sensitivity: The minimum detectable dose of dog eNOS is typically less than 3.9 U/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: </b<This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for eNOS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any eNOS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for eNOS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of eNOS bound in the initial step. The color development is stopped and the intensity of the color is measured.

75051779

Q9TUX8.1

Q9TUX8

133,046 Da

ACE Inhibitor Pathway 198583!!Arginine And Proline Metabolism Pathway 84003!!Arginine And Proline Metabolism Pathway 323!!Calcium Signaling Pathway 84100!!Calcium Signaling Pathway 459!!Disease Pathway 1016552!!Estrogen Signaling Pathway 799180!!Estrogen Signaling Pathway 799197!!HIF-1 Signaling Pathway 695209!!Hemostasis Pathway 1016763

Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets . Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. Cofactor: Heme group . Enzyme regulation: Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN

96 Strip Wells

915 USD