catalog number :
MBS701025
products type :
ELISA Kit
products full name :
Human Nuclear matrix protein 22, NMP-22 ELISA Kit
products short name :
Nuclear matrix protein 22, NMP-22
products name syn :
Human Nuclear matrix protein 22; NMP-22 ELISA Kit; Nuclear matrix protein 22; NMP-22
products gene name :
NMP-22
specificity :
This assay has high sensitivity and excellent specificity for detection of human NMP-22. No significant cross-reactivity or interference between human NMP-22 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and urine. Assay Type: Competitive. Detection Range: 0.1 ng/ml-40 ng/ml. Sensitivity: The minimum detectable dose of human NMP-22 is typically less than 0.04ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human NMP-22 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<6%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<11%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. Antibody specific for NMP-22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated NMP-22. A competitive inhibition reaction is launched between NMP-22 (Standards or samples) and HRP-conjugated NMP-22 with the pre-coated antibody specific for NMP-22. The more amount of NMP-22 in samples, the less antibody bound by HRP-conjugated NMP-22. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of NMP-22 in the sample. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells