Mouse neutrophil elastase, NE ELISA Kit | MyBioSource MBS701009 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse neutrophil elastase, NE ELISA Kit

catalog :

MBS701009

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS701009

products type :

ELISA Kit

products full name :

Mouse neutrophil elastase, NE ELISA Kit

products short name :

elastase 2, neutrophil

products name syn :

Mouse neutrophil elastase; NE ELISA Kit; ELA2; GE; HLE; HNE; NE; PMN-E; SCN1; bone marrow serine protease; elastase 2; neutrophil; granulocyte-derived elastase; leukocyte elastase; medullasin; neutrophil elastase; polymorphonuclear elastase; elastase 2; neutrophil

other names :

neutrophil elastase preproprotein; Neutrophil elastase; neutrophil elastase; elastase-2; medullasin; PMN elastase; leukocyte elastase; elastase 2, neutrophil; human leukocyte elastase; polymorphonuclear elastase; bone marrow serine protease; granulocyte-derived elastase; elastase, neutrophil expressed; Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase

products gene name :

ELA2

other gene names :

ELANE; ELANE; GE; NE; HLE; HNE; ELA2; SCN1; PMN-E; ELA2; HLE

uniprot entry name :

ELNE_HUMAN

reactivity :

Mouse

sequence length :

267

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse NE. No significant cross-reactivity or interference between mouse NE and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of mouse NE is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Assay Type: Sandwich

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NE bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4503549

ncbi acc num :

NP_001963.1

ncbi gb acc num :

NM_001972.2

uniprot acc num :

P08246

ncbi mol weight :

28,518 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); C-MYB Transcription Factor Network Pathway (138073); Collagen Degradation Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Systemic Lupus Erythematosus Pathway (83122); Systemic Lupus Erythematosus Pathway (534); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987); Amb2 Integrin Signaling Pathway (137945)

ncbi summary :

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]

uniprot summary :

Function: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.1 PublicationManual assertion based on experiment in:Ref.15. Catalytic activity: Hydrolysis of proteins, including elastin. Preferential cleavage: Val- -Xaa > Ala- -Xaa.

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage