catalog number :
MBS701008
products type :
ELISA Kit
products full name :
Mouse Interferon beta, IFN-beta/IFNB ELISA Kit
products short name :
interferon, beta 1, fibroblast
products name syn :
Mouse Interferon beta; IFN-beta/IFNB ELISA Kit; IFB; IFF; IFNB; MGC96956; interferon beta; interferon; beta 1; fibroblast
other names :
interferon beta; Interferon beta; interferon beta; interferon beta 1, fibroblast
products gene name :
IFNB1
other gene names :
Ifnb1; Ifnb1; Ifb; IFNB; IFN-beta; Ifb; Ifnb; IFN-beta
uniprot entry name :
IFNB_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse IFNB1. No significant cross-reactivity or interference between Mouse IFNB1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Antiviral defense
products description :
For Sample For the quantitative determination of mouse interferon ? (IFN-?/IFNB) concentrations in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. concentrations in serum, plasma, tissue homogenates. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IFN-?/IFNB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-?/IFNB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-?/IFNB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-?/IFNB bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_034640.1
ncbi gb acc num :
NM_010510.1
ncbi mol weight :
22,127 Da
ncbi pathways :
Chagas Disease (American Trypanosomiasis) Pathway (147810); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (970814); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response (BioCarta) Pathway (198283); Cytosolic DNA-sensing Pathway (125146); Cytosolic DNA-sensing Pathway (124833); EBV LMP1 Signaling Pathway (198354); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (1001326)
uniprot summary :
Function: Has antiviral, antibacterial and anticancer activities.
size4 :
10x96-Strip-Wells