catalog number :
MBS700983
products type :
ELISA Kit
products full name :
Rabbit apolipoprotein E, Apo-E ELISA Kit
products short name :
apolipoprotein E
products name syn :
Rabbit apolipoprotein E (Apo-E) ELISA Kit; AD2; LDLCQ5; LPG; MGC1571; apolipoprotein E3; Rabbit apolipoprotein E (Apo-E)
other names :
apolipoprotein E; Apolipoprotein E; apolipoprotein E; apo-E
products gene name :
APOE
other gene names :
APOE; APOE; Apo-E
uniprot entry name :
APOE_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rabbit Apo-E. No significant cross-reactivity or interference between rabbit Apo-E and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the quantitative determination of rabbit apolipoprotein E (Apo-E) concentrations in serum, plasma, tissue homogenates. Detection Range: 15.6 ng/ml -1000 ng/ml.
other info2 :
Sensitivity: The minimum detectable dose of rabbit Apo-E is typically less than 3.9 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: </b<This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apo-E. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for Apo-E. The competitive inhibition reaction is launched between with pre-coated Apo-E and Apo-E in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of Apo-E in the samples. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001076112.1
ncbi gb acc num :
NM_001082643.1
ncbi mol weight :
35,497 Da
ncbi pathways :
Alzheimer's Disease Pathway 83097!!Alzheimer's Disease Pathway 509!!Chylomicron-mediated Lipid Transport Pathway 106157!!HDL-mediated Lipid Transport Pathway 106158!!Lipid Digestion, Mobilization, And Transport Pathway 106111!!Lipoprotein Metabolism Pathway 106156!!Metabolism Pathway 477135!!Metabolism Of Lipids And Lipoproteins Pathway 160976!!Statin Pathway 198852
uniprot summary :
Function: Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.