ELISA/assay

Mouse pregnancy associated plasma protein A, PAPP-A ELISA Kit

MBS700979

96 Strip Wells

895 USD

ELISA Kit

Mouse pregnancy associated plasma protein A, PAPP-A ELISA Kit

pregnancy-associated plasma protein A, pappalysin 1

Mouse pregnancy associated plasma protein A; PAPP-A ELISA Kit; RP11-45A16.1; ASBABP2; DIPLA1; IGFBP-4ase; PAPA; PAPP-A; PAPPA1; aspecific BCL2 ARE-binding protein 2; differentially placenta 1 expressed protein; insulin-like growth factor-dependent IGF binding pro; pregnancy-associated plasma protein A; pappalysin 1

pappalysin-1; Pappalysin-1; pappalysin-1; IGF-dependent IGFBP-4 protease; insulin-like growth factor-dependent IGF-binding protein 4 protease; pregnancy-associated plasma protein A; Insulin-like growth factor-dependent IGF-binding protein 4 protease; IGF-dependent IGFBP-4 protease; IGFBP-4ase; Pregnancy-associated plasma protein A; PAPP-A

PAPPA

Pappa; Pappa; PAG1; PAPP-A; IGFBP-4ase; 8430414N03Rik; IGF-dependent IGFBP-4 protease; IGFBP-4ase; PAPP-A

PAPP1_MOUSE

Mouse

1624

This assay has high sensitivity and excellent specificity for detection of Mouse PAPPA. No significant cross-reactivity or interference between Mouse PAPPA and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 2.5 ng/ml-1000 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 2.5 ng/ml

Sample Volume: 50-100ul

Introduction: Pregnancy-associated plasma protein A, pappalysin 1, also known as PAPP-A, is a protein used in screening tests for Down syndrome. This protein is a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses (fetuses with an abnormal number of chromosomes). For example, low PAPPA may be seen in prenatal screening for Down syndrome. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific for PAPP-A antibody has been pre-coated onto a microplate. A competitive inhibition reaction is launched between HRP labeled PAPP-A and unlabeled PAPP-A (Standards or samples) with the antibody specific for PAPP-A. The substrate solutions are added to the wells, 3 respectively. And the color develops in opposite to the amount of PAPP-A bound in the initial step. The color development is stopped and the intensity of the color is measured.

59676577

NP_067337.1

NM_021362.1

Q8R4K8

181,318 Da

Metabolism Of Proteins Pathway 970583!!Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway 1000422

Function: Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. Isoform 2 cleaves IGFBP-4 very slowly compared to PAPP-A, but its ability to cleave IGFBP-5 is unaffected.1 PublicationManual assertion based on experiment in:Ref.2. Catalytic activity: Cleavage of the 135-Met- -Lys-136 bond in insulin-like growth factor binding protein (IGFBP)-4, and the 143-Ser- -Lys-144 bond in IGFBP-5. Cofactor: Binds 1 zinc ion per subunit

96 Strip Wells

895 USD